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Possibilité de culture de l'algue alimentaire Undaria Pinnatifida sur les côtes de France - L'ensemencement ArchiMer
Perez, Rene; Kaas, Raymond.
En 1971, un ostréiculteur de l'étang de Thau découvrait dans son parc à huîtres une algue qui lui paraissait étrange pour le lieu : il s' agissait en effet de l'espèce Undaria pinnatifida (MARVEY) SURINGAR (fig. 1), originaire du Japon, sans doute introduite involontairement sous forme de plantules ou de spores en même temps que le naissain d'huîtres importé d'Extrême-Orient.
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Ano: 1980 URL: http://archimer.ifremer.fr/doc/00046/15738/13140.pdf
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Les consequences de l'echouement de l'Amoco Cadiz" sur les peuplements algaux exploitables ArchiMer
Kaas, Raymond.
Introduction. The amount of hydrocarbons dumped into the sea is currently estimated at 6 to 16 million tons per year. This pollution comes from various sources: Chronic discharges associated with human activity such as oil tanker deballasting or discharges from coastal petroleum processing facilities; natural chronic discharges; accidental discharges like leaks coming from drilling well heads or oil tanker accidents. Natural spills happen, in general, in a relatively unobtrusive way and their effects are quickly diminished by significant dilution and rapid degradation of the various components. Oil spills, however, are characterised by a massive and occasional supply of hydrocarbons. The "Amoco Cadiz" shipwreck fits in with this last case and has seemed...
Tipo: Text Palavras-chave: Seaweeds; Pollution Effects; Marine Pollution; Oil Spill.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/publication-1950.pdf
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L'échouement de l'Amoco Cadiz : conséquences sur les peuplements algaux des côtes bretonnes ArchiMer
Kaas, Raymond.
Les 300 km du littoral breton touchés par les hydrocarbures de l'Amoco Cadiz sont parmi les plus productifs quant à l'exploitation des algues. L'ISTPM a suivi l'évolution des peuplements de Laminaria digitata et de Chondrus crispus, algues les plus utilisées par les industries locale et fait état des perturbations physiologiques engendrées par la pollution par les hydrocarbures.
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Ano: 1980 URL: https://archimer.ifremer.fr/doc/00593/70502/68653.pdf
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Technique de culture pour les cotes bretonnes de l'algue alimentaire Undaria pinnatifida. Tableau de marché - étude économique ArchiMer
Perez, René; Kaas, Raymond; Barbaroux, Olivier; Arbault, Suzanne; Le Bayon, Nicolas; Moigne, J.
Introduction Starting in 1986, the ANVAR contributed well appreciated financial support in order to set up an original seeding technique characterized by artificial seeding production in a laboratory. This advantageous new way of seeding, i.e. independency from nature, yield increase and true competitiveness compared to traditional methods, nevertheless requires strict adherence to a procedure progressively elaborated during numerous trials in laboratory as well as at sea. The program ended in July 1989 after testing the cultivation protocol on a 2 hectare exploitation scale, in order to better detect what could be improved to greatly increase productivity, facilitate the work of the harvester and to better adapt the operating mode to environmental...
Tipo: Text Palavras-chave: Economic survey; Brittany; Farming technique; Seaweed; Undaria pinnatifida; Etude économique; Bretagne; Technique de culture; Algue; Undaria pinnatifida.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-1659.pdf
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Essai de cryoconservation des gamétophytes de l'algue alimentaire Undaria pinnatifida (Laminariale) ArchiMer
Arbault, Suzanne; Renard, Philippe; Pérez, Rene; Kaas, Raymond.
Low temperature tolerance was determined for gametophytes of food alga Undaria pinnatifida according to various cryopreservation methods. Survival durations after storage in a medium containing 28% glycerol at −80 °C or in a 5-10% glycerol solution at −196 °C were only 9 and 4 days respectively. On the other hand, cooling at 5 °C/min. and storage at −15 °C for 15 minutes, without cryoprotective medium, did not reduce viability but this was highly decreased by storage for 15 minutes at −30 or −196 °C, with or without DMSO. The morphological cooling injuries were depigmentation and/or a contraction of the cells.
Tipo: Text Palavras-chave: Algue; Cryoconservation; Gamétophyte; Undaria pinnatifida; Alga; Cryopreservation; Gametophyte; Undaria pinnatifida.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00189/30071/28559.pdf
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La culture des algues marines dans le monde ArchiMer
Perez, René; Kaas, Raymond; Campello, Francois; Arbault, Suzanne; Barbaroux, Olivier.
Shouldn't Earth, the human planet, be called the algae planet? First appearing 3.5 billion years ago, algae have always been objective allies of life and humans. Brought about by algae, oxygen and the ozone layer allowed plant and animal life to develop in oceans and on continents. They offer spawning grounds and feeding grounds for marine fauna....
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/rapport-4402.pdf
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Optimisation de l'enrichissement nutritif pour la culture d' Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Loizel, Céline; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud.
La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. La solution d'enrichissement de Conway (SEC), élaborée en 1966 par Walne, est largement utilisée pour la production de T -iso en batch. Cependant, les apports nutritifs de cette solution d'enrichissement ne sont pas spécifiquement optimisés pour la culture de cette algue, participant à engendrer des coûts de production élevés (Valenzuala-Espinoza et al., 1999 ; Fabregas et al., 1986) et des effluents inépuisés. Dans les 15 dernières années, plusieurs auteurs sont parvenus à réduire le coût de solutions...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41519/40727.pdf
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Culture en continu de Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud.
La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est une micro algue marine classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. Aujourd'hui, de nombreuses écloseries de mollusques et de poissons l'utilisent en association avec d'autres espèces telles que Pavlova lutheri, Chaetoceros calcitrans et Skeletonema costatum. Isolée à Tahiti par JL Martin en 1975 (comm. pers.), cette algue s'est rapidement imposée dans la nutrition larvaire des crevettes et des mollusques en raison de sa croissance rapide en conditions tropicales et de ses qualités nutritionnelles, en relation avec sa forte teneur en acides gras...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41508/40716.pdf
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Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Kaas, Raymond; Baron, Regis.
Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and...
Tipo: Text Palavras-chave: Isochrysis; Cell quota; Photosynthesis; Steady state; Continuous culture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-597.pdf
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Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage ArchiMer
Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf
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Optimisation de la production en écloserie de mollusques : Mise au point d'un pilote industriel de production phytoplanctonique en continu et des techniques associées pour leur intégration en écloserie commerciale ArchiMer
Angeri, S.; Bougaran, Gael; Connan, Jean-paul; Jouin, Jean-claude; Kaas, Raymond; Le Dean, Loic; Legrand, J.; Loubiere, K.; Lukomska, Ewa; Olivo, Erell; Picquet, Marie-lise; Pruvost, J.; Robert, Rene.
Les travaux présentés dans le cadre de ce rapport d'activité rendent compte des avancées réalisées sur le plan (1) biologique, avec d'une part une étude sur le comportement du genre Chaetoceros en culture continue et, d'autre part, les résultats d'expérimentations sur les performances d'élevages larvaires réalisés sur la base de cultures de microalgues en continu, et (2) technologique, avec la continuité des actions de développement du photobioréacteur destiné aux écloseries commerciales ainsi que des périphériques indispensables à son intégration dans le secteur aquacole.
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41518/40726.pdf
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A New Process for Extracting Alginates from Laminaria digitata: Reactive Extrusion ArchiMer
Vauchel, Peggy; Kaas, Raymond; Arhaliass, Abdellah; Baron, Regis; Legrand, Julien.
Alginates are natural polysaccharides that are extracted from brown seaweeds and widely used for their rheological properties. The main step in the extraction protocol used in the alginate industry is that of alkaline extraction. A batch process is used for this step, which is time-, water-, and reactant-consuming. The possibility of extracting by reactive extrusion was investigated. The reactive extrusion process appeared to be more efficient than the batch process for the alkaline extraction of alginates from Laminaria digitata in several key ways: Time demand is reduced from about an hour to only few minutes, water and reactant requirements are divided by more than a factor 2, extraction yield is 15% higher (relative enhancement), and the rheological...
Tipo: Text Palavras-chave: Intrinsic viscosity; Reactive extrusion; Laminaria digitata; Alkaline extraction; Alginate.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6255.pdf
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Decrease in dynamic viscosity and average molecular weight of alginate from Laminaria digitata during alkaline extraction ArchiMer
Vauchel, Peggy; Arhaliass, Abdellah; Legrand, Jack; Kaas, Raymond; Baron, Regis.
Alginates are natural polysaccharides that are extracted from brown seaweeds and widely used for their rheological properties. The central step in the extraction protocol used in the alginate industry is the alkaline extraction, which requires several hours. In this study, a significant decrease in alginate dynamic viscosity was observed after 2 h of alkaline treatment. Intrinsic viscosity and average molecular weight of alginates from alkaline extractions 1-4 h in duration were determined, indicating depolymerization of alginates: average molecular weight decreased significantly during the extraction, falling by a factor of 5 between 1 and 4 h of extraction. These results suggested that reducing extraction time could enable preserving the rheological...
Tipo: Text Palavras-chave: Laminaria digitata; Intrinsic viscosity; Depolymerization; Average molecular weight; Alkaline extraction; Alginate.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4060.pdf
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Kinetics modeling of alginate alkaline extraction from Laminaria digitata ArchiMer
Vauchel, Peggy; Le Roux, Karine; Kaas, Raymond; Arhaliass, Abdellah; Baron, Regis; Legrand, Jack.
Alginates being depolymerized during their alkaline extraction, reducing extraction time could help producing higher rheological quality alginates. The purpose of the present work is to study fresh Laminaria digitata destructuration during alkaline extraction and its link to extraction kinetics. Both alginate extraction yield and mean diameter of algae particles were followed for different values of agitation level and initial size of algae pieces. Results highlighted the existence of a link between extraction yield and algal destructuration. Those elements and the specificity of L. digitata structure have been taken into account to propose a kinetics model based on a fluid-particle reaction with decreasing size particles. The model parameters have been...
Tipo: Text Palavras-chave: Model; Laminaria digitata; Alkaline extraction kinetics; Alginate.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6315.pdf
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Digital expression profiling of novel diatom transcripts provides insight into their biological functions ArchiMer
Maheswari, Uma; Jabbari, Kamel; Petit, Jean-louis; Porcel, Betina M.; Allen, Andrew E.; Cadoret, Jean-paul; De Martino, Alessandra; Heijde, Marc; Kaas, Raymond; La Roche, Julie; Lopez, P.; Martin-jezequel, Veronique; Meichenin, Agnes; Mock, Thomas; Parker, Micaela Schnitzler; Vardi, Assaf; Armbrust, E. Virginia; Weissenbach, Jean; Katinka, Michael; Bowler, Chris.
Background: Diatoms represent the predominant group of eukaryotic phytoplankton in the oceans and are responsible for around 20% of global photosynthesis. Two whole genome sequences are now available. Notwithstanding, our knowledge of diatom biology remains limited because only around half of their genes can be ascribed a function based onhomology-based methods. High throughput tools are needed, therefore, to associate functions with diatom-specific genes. Results: We have performed a systematic analysis of 130,000 ESTs derived from Phaeodactylum tricornutum cells grown in 16 different conditions. These include different sources of nitrogen, different concentrations of carbon dioxide, silicate and iron, and abiotic stresses such as low temperature and low...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12810/9758.pdf
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Dynamical modelling of a reactive extrusion process: Focus on residence time distribution in a fully intermeshing co-rotating twin-screw extruder and application to an alginate extraction process ArchiMer
Baron, Regis; Vauchel, Peggy; Kaas, Raymond; Arhaliass, Abdellah; Legrand, Jack.
The context of this study is the modelling of reactive extrusion process based on an alginate extraction protocol. Residence Time Distribution (RTD) is one important part to predict the kinetics of reactive compounds. A simple model is proposed to predict RTD in fully intermeshing co-rotating twin-screw extruders without reaction. This model, which can be easily extended to reactive case in a future work, is based on the extension of an axial dispersion model, including control parameters (screw speed and flow rate) and geometrical parameters (screw profile and die design). Simulations were performed for various operating and geometrical conditions so as to illustrate possibilities offered by the proposed model. Validation was conducted for two different...
Tipo: Text Palavras-chave: Alginate; Extraction; Extrusion; Mathematical modelling; Residence time distribution; Simulation; Laminaria digitata.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11592/8985.pdf
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Study on the microalgal pigments extraction process: Performance of microwave assisted extraction ArchiMer
Pasquet, Virginie; Cherouvrier, Jean-rene; Farhat, Firas; Thiery, Valerie; Piot, Jean-marie; Berard, Jean-baptiste; Kaas, Raymond; Serive, Benoit; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence...
Tipo: Text Palavras-chave: Pigments; Microalgae; Microwave; Extraction; Chlorophyll; Fucoxanthin; Carotene; MAE; VMAE.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14059/11533.pdf
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Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts ArchiMer
Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf
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Emergence d'une filière de culture de macro-algues en Bretagne et problème relatif à une espèce non indigène, le wakame (Undaria pinnatifida) ArchiMer
Antoine, Loic; Lemoine, Maud; Boulben, Sylviane; Kaas, Raymond; Laurans, Martial; Viard, Frédérique; Potin, Philippe.
La synthèse de l’expertise collégiale menée par l’Ifremer et la station biologique de Roscoff permet de préconiser le maintien des cultures de Undaria pinnatifida dans les zones déjà mises en culture à condition de se cantonner aux pratiques culturales des exploitants installés depuis longtemps, avec un suivi et sans expérimenter de nouvelles pratiques pour lesquelles il n’y a pas de recul. Là où des essais ont eu lieu en 2010 et 2011 (Projet Chaco), il serait nécessaire de faire rapidement un état des lieux de la présence ou l'absence d'Undaria pinnatifida en milieu naturel afin de mesurer l'impact de ces essais. En l’état actuel des connaissances il n’y a pas de données scientifiques qui permettent de garantir le respect de l’article 24 du R(CE) N°...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00086/19740/17380.pdf
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Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries ArchiMer
Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul.
Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal...
Tipo: Text Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf
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